多读论文可以丰富知识、拓展视野，启迪思维。现对我校2016-2020期间在发表的优质论文进行梳理，供参考。

筛选标准：被引频次、学科规范化引文影响力、学科百分位、主题显著度等指标（指标具体含义见文末）。

1. Comprehensive analysis of prognostic immune-related genes in the tumor microenvironment of cutaneous melanoma

作者：Yang, Sheng; Liu, Tong; Nan, Hongmei; Wang, Yan; Chen, Hao; Zhang, Xiaomei; Zhang, Yan; Shen, Bo; Qian, Pudong; Xu, Siyi; Sui, Jing; Liang, Geyu

发表期刊：JOURNAL OF CELLULAR PHYSIOLOGY   出版年：2020

摘要：Cutaneous malignant melanoma (hereafter called melanoma) is one of the most aggressive cancers with increasing incidence and mortality rates worldwide. In this study, we performed a systematic investigation of the tumor microenvironmental and genetic factors associated with melanoma to identify prognostic biomarkers for melanoma. We calculated the immune and stromal scores of melanoma patients from the Cancer Genome Atlas (TCGA) using the ESTIMATE algorithm and found that they were closely associated with patients’ prognosis. Then the differentially expressed genes were obtained based on the immune and stromal scores, and prognostic immune‐related genes further identified. Functional analysis and the protein–protein interaction network further revealed that these genes enriched in many immune‐related biological processes. In addition, the abundance of six infiltrating immune cells was analyzed using prognostic immune‐related genes by TIMER algorithm. The unsupervised clustering analysis using immune‐cell proportions revealed eight clusters with distinct survival patterns, suggesting that dendritic cells were most abundant in the microenvironment and CD8+ T cells and neutrophils were significantly related to patients’ prognosis. Finally, we validated these genes in three independent cohorts from the Gene Expression Omnibus database. In conclusion, this study comprehensively analyzed the tumor microenvironment and identified prognostic immune‐related biomarkers for melanoma.

2. Exosomal miRNA-19b-3p of tubular epithelial cells promotes M1 macrophage activation in kidney injury

作者：Lv, Lin-Li; Feng, Ye; Wu, Min; Wang, Bin; Li, Zuo-Lin; Zhong, Xin; Wu, Wei-Jun; Chen, Jun; Ni, Hai-Feng; Tang, Tao-Tao; Tang, Ri-Ning; Lan, Hui-Yao; Liu, Bi-Cheng

发表期刊：CELL DEATH AND DIFFERENTIATION   出版年：2020

摘要：Tubulointerstitial inflammation is a common characteristic of acute and chronic kidney injury. However, the mechanism by which the initial injury of tubular epithelial cells (TECs) drives interstitial inflammation remains unclear. This paper aims to explore the role of exosomal miRNAs derived from TECs in the development of tubulointerstitial inflammation. Global microRNA(miRNA) expression profiling of renal exosomes was examined in a LPS induced acute kidney injury (AKI) mouse model and miR-19b-3p was identified as the miRNA that was most notably increased in TEC-derived exosomes compared to controls. Similar results were also found in an adriamycin (ADR) induced chronic proteinuric kidney disease model in which exosomal miR-19b-3p was markedly released. Interestingly, once released, TEC-derived exosomal miR-19b-3p was internalized by macrophages, leading to M1 phenotype polarization through targeting NF-κB/SOCS-1. A dual-luciferase reporter assay confirmed that SOCS-1 was the direct target of miR-19b-3p. Importantly, the pathogenic role of exosomal miR-19b-3p in initiating renal inflammation was revealed by the ability of adoptively transferred of purified TEC-derived exosomes to cause tubulointerstitial inflammation in mice, which was reversed by inhibition of miR-19b-3p. Clinically, high levels of miR-19b-3p were found in urinary exosomes and were correlated with the severity of tubulointerstitial inflammation in patients with diabetic nephropathy. Thus, our studies demonstrated that exosomal miR-19b-3p mediated the communication between injured TECs and macrophages, leading to M1 macrophage activation. The exosome/miR-19b-3p/SOCS1 axis played a critical pathologic role in tubulointerstitial inflammation, representing a new therapeutic target for kidney disease.

3. Dysregulated N6-methyladenosine methylation writer METTL3 contributes to the proliferation and migration of gastric cancer

作者：Liu, Tong; Yang, Sheng; Sui, Jing; Xu, Si-Yi; Cheng, Yan-ping; Shen, Bo; Zhang, Yan; Zhang, Xiao-mei; Yin, Li-hong; Pu, Yue-pu; Liang, Ge-yu

发表期刊：JOURNAL OF CELLULAR PHYSIOLOGY   出版年：2020

摘要：Accumulating evidence implies that N6‐methyladenosine (m6A) methylation participated in the tumorigenesis of gastric cancer (GC). Here we synthetically analyzing the prognostic value and expression profile of seven m6A methylation‐relevant genes through silico analysis of sequencing data downloaded from The Cancer Genome Atlas, Kaplan–Meier plotter, and Gene Expression Omnibus database. We explored the methyltransferase‐like 3 (METTL3) expression in GC cell line and tumor tissues by reverse transcription quantitative polymerase chain reaction and western blot analysis. The m6A methylation status of total RNA was measured by m6A RNA methylation quantification kit. Small interfering RNA was used to establish METTL3 knockdown cell lines. We also measure the proliferation and migration capability GC cell. Furthermore, we detect the epithelial cell mesenchymal transition marker and m6A methylation level after METTL3 knock down. Our result revealed that METTL3 was significantly increased in GC tissues compared with control in big crowd data sets. Survival analysis showed that METTL3 serve as a poor prognostic factor for GC patients. The expression level of METTL3 gradually increased with the progress of tumor stage and grade. GFI1 is an important transcription factor associated with METTL3. We verified the up‐trend of METTL3 in messenger RNA and protein expression and observed a significant increase in the m6A methylation status of total RNA in the GC cells and tissues. METTL3 knockdown inhibited total RNA m6A methylation level, as well as cell proliferation and migration capacity. Moreover, METTL3 knockdown decreased α‐smooth muscle actin. Taken together, our finding revealed that m6A methylation writer METTL3 serve as an oncogene in tumorigenesis of GC.

4. Stress-Induced Metabolic Disorder in Peripheral CD4(+) T Cells Leads to Anxiety-like Behavior

作者：Fan, Ke-qi; Li, Yi-yuan; Wang, Hao-li; Mao, Xin-tao; Guo, Jin-xin; Wang, Fei; Huang, Ling-jie; Li, Yi-ning; Ma, Xiang-yu; Gao, Zheng-jun; Chen, Wei; Qian, Dan-dan; Xue, Wen-jin; Cao, Qian; Zhang, Lei; Shen, Li; Zhang, Long; Tong, Chao; Zhong, Jiang-yan; Lu, Wei; Lu, Ling; Ren, Ke-ming; Zhong, Guisheng; Wang, Yuan; Tang, Mingliang; Feng, Xin-hua; Chai, Ren-jie; Jin, Jin

发表期刊：CELL   出版年：2019

摘要：Physical or mental stress leads to neuroplasticity in the brain and increases the risk of depression and anxiety. Stress exposure causes the dysfunction of peripheral T lymphocytes. However, the pathological role and underlying regulatory mechanism of peripheral T lymphocytes in mood disorders have not been well established. Here, we show that the lack of CD4+ T cells protects mice from stress-induced anxiety-like behavior. Physical stress-induced leukotriene B4 triggers severe mitochondrial fission in CD4+ T cells, which further leads to a variety of behavioral abnormalities including anxiety, depression, and social disorders. Metabolomic profiles and single-cell transcriptome reveal that CD4+ T cell-derived xanthine acts on oligodendrocytes in the left amygdala via adenosine receptor A1. Mitochondrial fission promotes the de novo synthesis of purine via interferon regulatory factor 1 accumulation in CD4+ T cells. Our study implicates a critical link between a purine metabolic disorder in CD4+ T cells and stress-driven anxiety-like behavior.

5. Aptasensors for pesticide detection

作者：Liu, Mei; Khan, Arshad; Wang, Zhifei; Liu, Yuan; Yang, Gaojian; Deng, Yan; He, Nongyue

发表期刊：BIOSENSORS & BIOELECTRONICS   出版年：2019

摘要：Pesticide contamination has become one of the most serious problems of public health in the world, due to their wide application in agriculture industry to guarantee the crop yield and quality. The detection of pesticide residues plays an important role in food safety management and environment protection. However, the conventional detection methodologies cannot realize highly sensitive, se-lective and on-site detection, which limits their applications. Aptamers are short single-stranded oligonucleotides (RNA or DNA) se-lected by SELEX method, which can se-lectively bind to their targets with high affinity. Compared with the commonly used antibodies or enzymes in designing biosensors, aptamers exhibit better stability, low molecular weight, easy modification and low cost, and were regarded as excellent candidates for developing aptasensors for pesticide detection. In this review, application of aptamers for pesticide detection was reviewed. Firstly, aptamers specifically bind to various pesticides were first summarized. Secondly, the progresses and highlights of developing aptasensors for highly-sensitive and se-lective detection of pesticide residues were systematically provided. Finally, the present challenges and future perspectives for developing novel highly-effective aptasensor for the detection of pesticide residues were discussed.

6. Quantitative and ultrasensitive detection of multiplex cardiac biomarkers in lateral flow assay with core-shell SERS nanotags

作者：Zhang, Di; Huang, Li; Liu, Bing; Ni, Haibin; Sun, Liangdong; Su, Enben; Chen, Hongyuan; Gu, Zhongze; Zhao, Xiangwei

发表期刊：BIOSENSORS & BIOELECTRONICS   出版年：2018

摘要：Rapid and sensitive quantification of multiplex proteins in a wide concentration range is challenging in high throughput analysis. Herein, we proposed a lateral flow assay (LFA) based on core-shell surface enhanced Raman scattering (SERS) nanotags for multiplex and quantitative detection of cardiac biomarkers for the early diagnosis of acute myocardial infarction (AMI). In practice, Raman dyes (RDs) were embedded into the interior-gap of silver core and gold shell nanoparticles (NPs) to form SERS nanotags as labels instead of gold colloids and three test lines were employed in the strip for the detection of three cardiac biomarkers, Myo, cTnI, and CK-MB, respectively. Due to the amplified signal of the SERS nanotags and the high surface area to volume ratio (SVR) of porous nitrocellulose (NC) membrane, ultrasensitive quantification of protein markers with wide linear dynamic range (LDR) was realized, which is crucial for the quick detection of multiplex biomarkers in the same sample without pretreatments at bedsides. This method makes it possible for LFA in point of care testing (POCT) to be comparable with chemiluminescence immunoassay (CLIA) used in labs.

7. Novel insight into circular RNA HECTD1 in astrocyte activation via autophagy by targeting MIR142-TIPARP: implications for cerebral ischemic stroke

作者：Han, Bing; Zhang, Yuan; Zhang, Yanhong; Bai, Ying; Chen, Xufeng; Huang, Rongrong; Wu, Fangfang; Leng, Shuo; Chao, Jie; Zhang, John H.; Hu, Gang; Yao, Honghong

发表期刊：AUTOPHAGY   出版年：2018

摘要：Circular RNAs (circRNAs) are highly expressed in the central nervous system and are involved in the regulation of physiological and pathophysiological processes. However, the potential role of circRNAs in stroke remains largely unknown. Here, using a circRNA microarray, we showed that circular RNA Hectd1 (circHectd1) levels were significantly increased in ischemic brain tissues in transient middle cerebral artery occlusion (tMCAO) mouse stroke models and further validated this finding in plasma samples from acute ischemic stroke (AIS) patients. Knockdown of circHectd1 expression significantly decreased infarct areas, attenuated neuronal deficits, and ameliorated astrocyte activation in tMCAO mice. Mechanistically, circHECTD1 functions as an endogenous MIR142 (microRNA 142) sponge to inhibit MIR142 activity, resulting in the inhibition of TIPARP (TCDD inducible poly[ADP-ribose] polymerase) expression with subsequent inhibition of astrocyte activation via macroautophagy/autophagy. Taken together, the results of our study indicate that circHECTD1 and its coupling mechanism are involved in cerebral ischemia, thus providing translational evidence that circHECTD1 can serve as a novel biomarker of and therapeutic target for stroke.

8. Recent progresses in DNA nanostructure-based biosensors for detection of tumor markers

链接地址：https://www.sciencedirect.com/science/article/pii/S0956566318301544

作者：Huang, Rongrong; He, Nongyue; Li, Zhiyang

发表期刊：BIOSENSORS & BIOELECTRONICS   出版年：2018

链接地址：https://www.sciencedirect.com/science/article/pii/S0956566319300272

摘要：DNA has emerged as a promising biomaterial for assembling a variety of nanostructures based on its programmable base pairing. It also has other remarkable properties including stability, prominent biocompatibility, and can easily be modified with functional groups for further applications. In the past few decades, researchers have established various design rules and assembly technologies to improve the stability and complexity of DNA nanostructures. The detection of cancer-associated biomarkers has significant importance in identifying patients with different clinical stages and also in developing adaptive therapeutic strategies. Due to their unique advantages, DNA nanostructures can be designed to serve as universal units to form biosensors for the detection of tumor biomarkers. In this review, we first present a brief introduction of the development of structural DNA nanotechnology. Then we summarize recent strategies for DNA nanostructure-based optical, electrochemical and mass sensitive biosensors in cancer detection. Finally, we discuss the challenges and opportunities these technologies provide.

9. Gold nanoparticles superlattices assembly for electrochemical biosensor detection of microRNA-21

作者：Tian, Liang; Qian, Kun; Qi, Jinxu; Liu, Qinyao; Yao, Chen; Song, Wei; Wang, Yihong

发表期刊：BIOSENSORS & BIOELECTRONICS   出版年：2018

摘要：Gold nanoparticles (AuNPs) superlattice and small molecule dyes such as toluidine blue have remarkable effect on signal amplification. In this report, a label-free and simple electrochemical microRNA biosensor is developed by employing toluidine blue (TB) as a redox indicator and AuNPs superlattice as a support material. Conductive polymer, polypyrrole coated AuNPs was self-assembled to form a superlattice which exhibited the most close-packed type thereby producing the maximum current. The successful immobilization of the single strand RNA (ss-RNA) probe and hybridization with the target microRNA sequence were confirmed by electrochemical cyclic voltammetry (CV) methods as well as differential pulse voltammetry (DPV) technique, which was used to determine the oxidation peak current of TB under optimal condition. TB with efficient signal amplification was applied in microRNA biosensor for the first time. By employing this strategy, microRNA can be detected in a range from 100 aM to 1 nM with a relatively low detection limit of 78 aM. Alongside the outstanding sensitivity and se-lectivity, this nanobiosensor had great reproducibility and showed a remarkable response in the real sample analysis with serum samples. In conclusion, the proposed electrochemical nanobiosensor could be clinically useful in the early detection of the breast cancer by direct detection of the serum microRNA-21 in real clinical samples without sample preparation, RNA extraction and/or amplification.

10. hsa_circ_0013958: a circular RNA and potential novel biomarker for lung adenocarcinoma

作者：Zhu, Xiaoli; Wang, Xiyong; Wei, Shuzhen; Chen, Yan; Chen, Yang; Fan, Xiaobo; Han, Shuhua; Wu, Guoqiu

发表期刊：FEBS JOURNAL   出版年：2017

摘要：Circular RNAs (circRNAs) are associated with cancer progression and metastasis, although little is known about their role in lung adenocarcinoma (LAC). In the present study, microarrays were first used to screen for tumour‐specific circRNA candidates in LAC tissue. Thirty‐nine circRNAs were found to be up‐regulated and 20 were down‐regulated (fold change > 2.0). Among them, hsa_circ_0013958 was further confirmed to be up‐regulated in all of the LAC tissues, cells and plasma. In addition, hsa_circ_0013958 levels were associated with TNM stage (P = 0.009) and lymphatic metastasis (P = 0.006). The area under the receiver operating characteristic curve was 0.815 (95% confidence interval = 0.727–0.903; P < 0.001). In addition, to further illustrate the bioactivities of hsa_circ_0013958 in LAC, siRNA‐mediated inhibition of hsa_circ_0013958 was performed in vitro. The results showed that hsa_circ_0013958 promoted cell proliferation and invasion and inhibited cell apoptosis in LAC. Moreover, hsa_circ_0013958 was identified as a sponge of miR‐134, and thus it up‐regulated oncogenic cyclin D1, which plays a pivotal role in the development of non‐small cell lung cancer. In conclusion, our results suggested that hsa_circ_0013958 could be used as a potential non‐invasive biomarker for the early detection and screening of LAC.

Tips：指标解释 被引频次：该篇文献被引用的总次数。 学科规范化的引文影响力：等于该篇文献的实际被引次数除以同文献类型、同出版年、同学科领域文献的期望被引次数（期望被引次数即篇均被引频次）。大于1则表示该篇文献的被引表现高于全球平均水平。 学科百分位：一篇文献的百分位是通过建立同出版年、同学科领域、同文献类型的所有文献的被引频次分布（将论文按照被引频次降序排列），并确定低于该论文被引次数的论文的百分比获得的。如果一篇论文的百分位值为1，则该学科领域、同出版年、同文献类型中99%的论文的引用次数都低于该论文。 主题显著度：主题显著度由三个指标计算出，三个指标分别是：该主题中所有文献在第n年到n-1年的引用次数，该主题中所有文献第n年到n-1年在SCOPUS数据库中的浏览次数，该主题中所有文献第n年的CiteScore（衡量期刊影响力的指标）的平均值。